Analysis Note

ControlPC12 cell lysate

Application

Anti-GluR-2 Antibody, C-terminus detects level of GluR-2 & has been published & validated for use in WB.

Research CategoryNeuroscience

Research Sub CategoryNeurotransmitters & Receptors

Immunoprecipitation: A previous lot of this antibody successfully immoprecipitated GluR-2 from a brain tissue lysate, as reported by an independent laboratory.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Glutamate receptors (GluRs) can be categorized as ionotropic or metabotropic and subcatergorized by their agonist preferences (NMDA, AMPA or Kainic acid). There are four types of AMPA selective GluR subunits (GluR1, GluR2, GluR3 and GluR4). Tetrameric or pentameric combinations of different subunits contributes to the functional diversity of AMPA receptors. In general, AMPA receptors mediate fast synaptic current at most excitatory synapses, with stoichiometry characterized by subtype composition. Although subunit composition of AMPA receptors varies, they must contain at least one edited GluR2 subunit to be calcium impermeable. The critical residue controlling calcium permeability is in the pore loop region. In GluR1, GluR3, and GluR4, this positionis occupied by a Gln residue. In GluR2, it is occupied by an Arg residue. It has been shown experimentally that the presence of Arg in this position blocks CA2+ ion permeability, while a Gln does not. Relative calcium permeability in AMPA receptor channels may be significant in pathological neurotoxic damage and long term changes in nervous system responses.

Immunogen

Epitope: C-terminus

Recombinant protein corresponding to the C-terminus of rat GluR-2.

Physical form

Rabbit polyclonal serum containing 0.05% sodium azide.

Unpurified

Quality

Evaluated by Western Blot in PC12 cell lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected GluR-2 on 10 µg of PC12 cell lysate.

Specificity

This antibody recognizes GluR-2 at the C-terminus.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~106 kDa Observed